96000 Drugs of Abuse Testing in Urine – with Reaction Vials

MassTox® Drugs of Abuse Testing in Urine with Reaction Vials

Order no.: 96000
400 Tests
  • Amphetamines
  • Barbiturates
  • Benzodiazepines
  • Booster
  • Cannabinoids
  • Cocaine
  • Opiates/Opioids
  • Other drugs of abuse
  • Z-Drugs

More than 100 drugs and metabolites
Target screening and quantitative confirmation within the same run
Sample prep with reaction vials

Description

Clinical relevance

Drug abuse is one of the most pressing public health issues worldwide, including the use of both illegal drugs and prescription medicines. Cannabis is by far the most commonly consumed illegal drug in Europe. Distant runners-up are the stimulants cocaine, MDMA and amphetamines. Within the class of opioids, heroin continues to be the most widely abused drug.

Drug of abuse testing in forensic medicine can establish both illicit drug use and whether crimes were committed under the influence of drugs. During the treatment of drug addicts, it is also performed to verify the abstinence of illicit drugs during replacement and maintenance therapy. In occupational medicine, drug screening is used to check for abstinence and to determine if a patient is fit to drive. Accurate testing can also help to save lives when someone is intoxicated.

 

Product advantages

  • Assay includes 108 drugs and metabolites
  • Target screening and quantitative confirmation within the same run
  • Run time of 12 minutes
  • Simple sample preparation combined with high selectivity and sensitivity
  • Internal standards for 98 analytes
  • With reaction vials or with 96 well plate

 

MassTox® Drugs of Abuse Testing is an LC-MS/MS-based assay that helps to increase speed of analysis and eliminates the need for developing and maintaining in-house methods or specific QC materials. The test is available with reaction vials or 96 well plates and allows the target screening of 108 drugs and metabolites. The confirmatory analysis can also be performed within the same run. It provides a significantly higher selectivity and sensitivity than current immunoassays and is designed to work below cut-offs applied in forensic labs. Semi-quantitative statements are also possible.

The assay includes isotopically labelled internal standards for nearly all analytes and allows the separation of all substances in a single chromatographic separation of approximately 12 minutes. The high stability of the 6PLUS1® Multilevel Calibrator Set and MassCheck® controls with three levels ensure reliable results with highest precision. A specific urine control with a carefully selected set of glucuronide metabolites allows controlling the proper functioning of the hydrolysis process. This Hydrolysis Control can also be used in laboratories working with their own developed methods and enzymes.

 

108 Drugs and Metabolites

Amphetamines
Amphetamine
BDB
Butylone
2C-B
2C-I
Cathinone
MBDB
MDA
MDEA
MDMA
MDPV
Mephedrone
Methamphetamine
Methaqualone
Methylone
Methylphenidate
PMA
Ritalinic Acid

 

Benzodiazepines
Alprazolam
7-Aminoclonazepam
7-Aminoflunitrazepam
7-Aminonitrazepam
Bromazepam
Brotizolam
Chlordiazepoxide
Clobazam
Clonazepam
Demoxepam
Desalkylflurazepam
Desmethylflunitrazepam
Diazepam
Estazolam
Flunitrazepam
Flurazepam
Lorazepam
Lormetazepam
Medazepam
Midazolam
Nitrazepam
Norclobazam
Nordiazepam
α-OH-Alprazolam
3-OH-Bromazepam
α-OH-Midazolam
α-OH-Triazolam
Oxazepam
Prazepam
Temazepam
Triazolam

Opiates/Opioids
Acetylcodeine
Buprenorphine
Codeine
Dihydrocodeine
EDDP
Fentanyl
Hydrocodone
Hydromorphone
Meconin
Meperidine
Methadone
6-Monoacetylmorphine
Morphine
Naloxone
Naltrexone
Norbuprenorphine
Norcodeine
Norfentanyl
Normeperidine
Nortapentadol
Nortilidine
O-Desmethyltramadol
Oxycodone
Oxymorphone
Papaverine
Propoxyphene
Sufentanil
Tapentadol
Thebaine
Tilidine
Tramadol

Booster
Gabapentin
Pregabalin
Promethazine
Quetiapine

Cannabinoids
THC-COOH

Cocaine
Benzoylecgonine
Cocaethylene
Cocaine
Norcocaine

Z-Drugs
Zaleplon
Zolpidem
Zopiclone

Barbiturates
Allobarbital
Amobarbital
Barbital
Butalbital
Hexobarbital
Pentobarbital
Phenobarbital
Secbutabarbital
Secobarbital
Thiopental

Others
Ketamine
LSD
Mescaline
Norketamine
O-H-LSD
PCP

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Number of Tests

400

Parameter

Amphetamines, Barbiturates, Benzodiazepines, Booster, Cannabinoids, Cocaine, Opiates/Opioids, Other drugs of abuse, Z-Drugs

Analysis Time

12.5 min

Limit of quantification

down to 13 ng/l

Linearity

for all analytes: from 0.25 to 30 x of cut-off value

Recovery

85–115 %

Intraassay

CV = 1–9 %

Interassay

CV = 1–11 %

Specimen

Urine

Pre-analytic Treatment

Stability of specimen: according to the Swiss Guidelines Committee for Drugs of Abuse Testing (SCDAT), samples are stable for seven days at +2 to +8 °C and for six months at -18 °C.

Sample Preparation

  • Pipette 50 µl sample/calibrator/MassCheck® control into a reaction vial.
  • Add 10 µl Internal Standard Mix.
  • Add 40 µl Enzyme Buffer Mix, mix briefly (vortex).
  • Incubate for 120 minutes at 45 ° C.
  • Add 100 µl Precipitation Reagent, mix briefly (vortex) and centrifuge for 5 min.
  • In an autosampler vial, dilute 100 µl supernatant with 150 µl Dilution Buffer, mix briefly (vortex).
  • Inject ≤ 20 µl into the LC-MS/MS system.

Injection Volume

2–20 μl

Flow rate

0.4 ml/min

Column temperature

30 °C

Ionisation

ESI positive and negative

MS/MS-Mode

MRM

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Calibrators

Calibrators available separately

Controls