54930-500 HPLC kit OneStep CDT serum fast elution

One Step CDT in Serum with Fast Elution - HPLC

Order no.: 54930/500
500 Tests
  • Asialotransferrin
  • Disialotransferrin
  • Pentasialotransferrin
  • Tetrasialotransferrin
  • Trisialotransferrin

With ready-to-use pre-mixed tubes
Only one pipetting step
Detects genetic variants

Description

Clinical relevance

CDT (carbohydrate-deficient transferrin) is a lab marker showing the highest specificity for chronic alcohol abuse. CDT values increase significantly with the daily intake of more than 60 g ethanol (the equivalent of 0.75 l wine) per day over a period of two weeks. The values return to normal within two weeks in case of alcohol abstention. This makes CDT an excellent parameter for continuous monitoring in withdrawal programmes or for assessments in forensic or occupational medicine.

Unlike other biochemical parameters like γ-GT or MCV, liver diseases will not cause any false-positive results.

 

Product advantages

  • Very easy sample preparation with pre-mixed tubes
  • Only one pipetting step required
  • Detection of genetic variants

 

This Chromsystems kit allows for the fast and reliable measurement of CDT in serum using HPLC and UV detection. Sample preparation with premixed tubes is performed in a single pipetting step, making it fast and convenient.

The CDT is displayed as an area percentage of total transferrin, so it remains unaffected by fluctuations in the concentration of transferrin in serum. Optimised chromatographic separation of transferrin isoforms also allows for the reliable detection of known genetic variants.

A manual method with automated sample preparation is also available as an alternative for greater sample throughput.

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

500

Parameter

Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin

Analysis Time

10 min

Limit of quantification

approx. 0.5 % Disialotransferrin

Intraassay

CV = 2.4 % (with 3.3 % Disialo)

Interassay

CV = 3.2 % (with 3.3 % Disialo)

Column temperature

Ambient (~25 °C)

Flow rate

Detection: 1.5 ml/min, Rinsing: 2.5 ml/min

Specimen

Serum

Pre-analytic Treatment

Specimens
Serum (no anticoagulants), no EDTA or heparin plasma!

Stability of specimens
For longer transport send cooled at +2 to +8 °C, deep freezing is possible.

Sample Preparation

  • Add 100 µl serum to a Pre-mix Tube.
  • Vortex for 60 sec.
  • Incubate 30 min at +2 to +8°C.
  • Centrifuge 10 min at 9000 x g.
  • Inject 150–200 µl of the undiluted supernatant into the HPLC system.

Sample Stability

6 days at ambient temperature

Injection Volume

150-200 µl

Additional Info

For the Chromsystems HPLC analysis of CDT in serum with fast elution chromatography a ternary HPLC gradient system with UV detection is necessary.

Wavelength

460 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase A


Order no.: 54931

Mobile Phase B


Order no.: 54932

Mobile Phase C


Order no.: 54933

Pre-mixed Tubes


Order no.: 54934

Controls

Controls available separately

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