69000 MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine

MassChrom® Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine - LC-MS/MS

Order no.: 69000
400 Tests
  • Ethyl glucuronide (EtG)
  • Ethyl sulfate (EtS)

Very high column stability
Validated according to GTFCh guidelines
One step sample prep
Reliable determination also near cut-off values

Description

Clinical relevance

Alcohol is one of the world’s most frequently consumed drugs and is quickly absorbed in the small intestine of humans. It is metabolized in the liver to acetaldehyde and then to acetic acid. The elimination is quite fast, subsequently, the detection period of the alcohol itself is often limited to approximately 12 hours. However, less than 0.1% of the consumed alcohol is glucurono- or sulpho-conjugated, which is excreted as ethyl glucuronide (EtG) and ethyl sulphate (EtS). Both are detectable for a sustained period in comparison to ethanol itself, making them reliable and specific biomarkers for the clarification of alcohol consumption. Thus, EtG and EtS close the gap between short-term markers such as ethanol and long-term markers such as CDT.

 

Product benefits

  • Very high column stability
  • Validated according to GTFCh guidelines
  • One step sample preparation
  • Reliable determination also near cut-off values

 

The MassChrom® kit from Chromsystems enables reliable and precise determination of EtG and EtS in urine. This assay has been comprehensively validated according to the guidelines of the German Society of Toxicological and Forensic Chemistry (GTFCh) for quality assurance and enables the determination of both parameters with one sample preparation and in one single analysis time of 6 minutes. The straightforward and efficient sample preparation consists of a single pipetting step that includes the instrument-specific dilution. The human matrix-based 6PLUS1® Multilevel Calibrator Set and the MassCheck® controls ensure the precision and accuracy of the results.

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Number of Tests

400

Parameter

Ethyl glucuronide (EtG), Ethyl sulfate (EtS)

Analysis Time

6 min

Limit of quantification

EtG: 35 µg/l
EtS: 20 µg/l

Recovery

EtG: 97-100 %; EtS: 95-98 %

Intraassay

CV < 3 %

Interassay

CV < 4 %

Gradient

0.00–3.00 min, 0 % Mobile Phase B
3.01–5.50 min, 100 % Mobile Phase B
5.51–6.00 min, 0 % Mobile Phase B

Information

It is recommended to set the scan time to a value that allows a minimum of 10 data points over the whole peak width.

Flow rate

0.5-1.0 ml/min

Specimen

Urine

Sample Preparation

Place 50 µl sample/calibrator/MassCheck® control in autosampler vial.
Dilute as required by the instrument with Master Mixture (minimum dilution of 1:5).
Vortex briefly.
Inject up to 20 µl into the LC-MS/MS system.

Master Mixture
Example for 100 samples and a target dilution of 1:10: Add 2.5 ml Internal Standard Mix to 42.5 ml Dilution Buffer. Add 450 µl of this mixture to 50 µl sample.

Sample Stability

Samples are stable up to 5 days at +2 to +8°C. For longer storage up to 3 months keep samples frozen below -18°C.

Injection Volume

up to 20 µl

Ionisation

ESI negative

MS/MS-Mode

MRM

Additional Info

It is recommended to set the scan time to a value that allows a minimum of 10 data points over the whole peak width.

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Calibrators

Controls

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