CDT in Serum - ternary gradient systems - HPLC

CDT in Serum - ternary gradient systems - HPLC

   
  • Asialotransferrin
  • Disialotransferrin
  • Pentasialotransferrin
  • Tetrasialotransferrin
  • Trisialotransferrin

Order no.: 54030, 54030/500
100 / 500 Tests

Sample preparation: just 2 pipetting steps
Long column lifetime
Low cost per test
Reference method for CDT analyses: currently HPLC

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Description

Clinical relevance

CDT (carbohydrate-deficient transferrin) is a lab marker showing the highest specificity for chronic alcohol abuse. CDT values increase significantly with the daily intake of more than 60 g ethanol (the equivalent of 0.75 l wine) per day over a period of two weeks. The values return to normal within two weeks in case of alcohol abstention. This makes CDT an excellent parameter for continuous monitoring in withdrawal programmes or for assessments in forensic or occupational medicine.

Unlike other biochemical parameters like γ-GT or MCV, liver diseases will not cause any false-positive results.

 

Product advantages

  • Reference method for accurate CDT determination
  • Sample preparation in only 2 steps
  • Long column lifetime
  • Low cost

 

This Chromsystems kit allows for the fast and reliable measurement of CDT in serum using HPLC and UV detection. The results are displayed as an area percentage of total transferrin, so they remain unaffected by fluctuations in transferrin concentration. Optimised chromatographic separation of transferrin isoforms also allows for the reliable detection of genetic variants of transferrin.

Alternative methods using pre-mixed tubes or automated sample preparation for higher sample throughput are also available.

Chromatogram

Technical Data

Method of Analysis

HPLC

Parameter

Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin

Analysis Time

20 min

Limit of quantification

0.5 % Disialotransferrin

Linearity

< 12 % Disialotransferrin

Recovery

99 % (ternary gradient)

Intraassay

CV 6.2 % (ternary gradient)

Interassay

CV 3.5 % (ternary gradient)

Column temperature

ambient (~25 °C)

Flow rate

1.5 ml/min

Specimen

Serum (no anticoagulants, no EDTA or heparin plasma)

Pre-analytic Treatment

Time of sample drawing

  • Has no influence on the analysis
  • Fasting serum preferred

Sample Preparation

  • Place 200 µl serum in a reaction vial.
  • Add 100 µl reaction mix*.
  • Vortex for 60 s.
  • Incubate 30 min at +2 bis +8 °C.
  • Centrifuge 10 min at 9000 g.
  • Mix 200 µl supernatant with 400 µl water.
  • Inject 200 µl of this mixture into the HPLC system.

*Composition of the reaction mix per specimen: 25 µl Neutralisation Buffer, 25 µl Stabilisation Buffer, 25 µl Precipitation Reagent 1 and 25 µl Precipitation Reagent 2.
Independent of the number of specimens you are analyzing you have to prepare this "master mix" only once and can add it to the samples e.g. by a multipette.

Sample Stability

At ambient temperature up to 30 h. Keep cool for longer transport and deep-freezing is acceptable.

Injection Volume

200 µl

Additional Info

For the Chromsystems HPLC analysis of CDT in serum any ternary HPLC gradient system with UV detection is suitable.

Wavelength

460 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase A


Order no.: 54031

Mobile Phase B


Order no.: 54032

Mobile Phase C


Order no.: 54033

Neutralisation Buffer


Order no.: 54025

Stabilisation Buffer


Order no.: 54026

Precipitation Reagent 1


Order no.: 54027

Precipitation Reagent 2


Order no.: 54028

Reaction vials


Order no.: 3006

Controls

Controls available separately

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