CDT in Serum - binary gradient systems - HPLC

CDT in Serum - binary gradient systems - HPLC

   
  • Asialotransferrin
  • Disialotransferrin
  • Pentasialotransferrin
  • Tetrasialotransferrin
  • Trisialotransferrin

Order no.: 54020, 54020/500
100 / 500 Tests

Reference method for CDT analyses: currently HPLC
Sample preparation: just 2 pipetting steps
Long column lifetime
Low cost per test

 

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Description

Clinical relevance

CDT (carbohydrate-deficient transferrin) is a lab marker showing the highest specificity for chronic alcohol abuse. CDT values increase significantly with the daily intake of more than 60 g ethanol (the equivalent of 0.75 l wine) per day over a period of two weeks. The values return to normal within two weeks in case of alcohol abstention. This makes CDT an excellent parameter for continuous monitoring in withdrawal programmes or for assessments in forensic or occupational medicine.

Unlike other biochemical parameters like γ-GT or MCV, liver diseases will not cause any false-positive results.

 

Product advantages

  • Reference method for accurate CDT determination
  • Sample preparation in only 2 steps
  • Long column lifetime
  • Low cost

 

This Chromsystems kit allows for the fast and reliable measurement of CDT in serum using HPLC and UV detection. The results are displayed as an area percentage of total transferrin, so they remain unaffected by fluctuations in transferrin concentration. Optimised chromatographic separation of transferrin isoforms also allows for the reliable detection of genetic variants of transferrin.

Alternative methods using pre-mixed tubes or automated sample preparation for higher sample throughput are also available.

Chromatogram

Technical Data

Method of Analysis

HPLC

Parameter

Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin

Analysis Time

22 min

Limit of quantification

0.5 % Disialotransferrin

Linearity

< 12 % Disialotransferrin

Recovery

103 %

Intraassay

CV ≤ 7.7 %

Interassay

CV ≤ 4.6 %

Gradient

Binary

Column temperature

ambient (~25 °C)

Flow rate

1.5 ml/min (analysis); 2.0 ml/min (column wash)

Specimen

Serum (no anticoagulants, no EDTA or heparin plasma)

Pre-analytic Treatment

Time of sample drawing has no influence on the analysis, fasting serum preferred. Samples should be cooled during transport.

 

Sample Preparation

  • Place 200 µl serum in a reaction vial.
  • Add 100 µl reaction mix*.
  • Vortex for 60 s.
  • Incubate 30 min at +2 bis +8 °C.
  • Centrifuge 10 min at 9000 x g.
  • Dilute 200 µl supernatant with 400 µl ultrapure water (HPLC grade).
  • Inject 200 µl into the HPLC system.

*Preparation of the reaction mix: mix 25 µl each of Neutralisation Buffer, Stabilisation Buffer, Precipitation Reagent 1 and 2. Independent of the number of specimens you are analyzing you can prepare this "master mix" only once and can add it to the samples e.g. by a multipette.

Sample Stability

Samples are stable at room temperature up to 30 h, at +2 to +8 °C up to 1 week. For longer storage keep them frozen below –18 °C.

Injection Volume

200 µl

Additional Info

For the Chromsystems HPLC analysis of CDT in serum any binary HPLC gradient system with UV detection is suitable.

Wavelength

460 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase A


Order no.: 54021

Mobile Phase B


Order no.: 54022

Neutralisation Buffer


Order no.: 54025

Stabilisation Buffer


Order no.: 54026

Precipitation Reagent 1


Order no.: 54027

Precipitation Reagent 2


Order no.: 54028

Reaction vials


Order no.: 3006

Controls

Controls available separately

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