54730-F HPLC kit automated CDT serum 96 well filter plates

Automated CDT in serum with 96 Well Filter Plate - HPLC

Order no.: 54730/F
6 x 96 Tests
  • Asialotransferrin
  • Disialotransferrin
  • Pentasialotransferrin
  • Tetrasialotransferrin
  • Trisialotransferrin

96 samples in 75 minutes
Suitable for a range of liquid handling devices
Analysis detects genetic variants

Description

Clinical relevance

CDT (carbohydrate-deficient transferrin) is a lab marker showing the highest specificity for chronic alcohol abuse. CDT values increase significantly with the daily intake of more than 60 g ethanol (the equivalent of 0.75 l wine) per day over a period of two weeks. The values return to normal within two weeks in case of alcohol abstention. This makes CDT an excellent parameter for continuous monitoring in withdrawal programmes or for assessments in forensic or occupational medicine.

Unlike other biochemical parameters like γ-GT or MCV, liver diseases will not cause any false-positive results.

 

Product advantages

  • Automated sample preparation
  • 96 samples in 75 minutes

 

This Chromsystems kit allows for the fast and reliable measurement of CDT in serum using HPLC and UV detection. Automated sample preparation can save a significant amount of time and resources for larger sample batches. 96 samples can be prepared within 75 minutes.

The CDT is displayed as an area percentage of total transferrin, so it remains unaffected by fluctuations in the concentration of transferrin in serum. Optimised chromatographic separation of transferrin isoforms also allows for the reliable detection of known genetic variants.

A manual standard method and one with pre-mixed tubes are available as alternatives.

 

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

6 x 96

Parameter

Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin

Analysis Time

10 min

Limit of quantification

approx. 0.5 % Disialotransferrin

Intraassay

CV = 2.7 % (with 3.4 % Disialo)

Interassay

CV = 5.7 % (with 3.4 % Disialo)

Column temperature

Ambient (~25 °C)

Flow rate

Detection: 1.5 ml/min, Rinsing: 2.5 ml/min

Specimen

Serum

Pre-analytic Treatment

Specimens
Serum (no anticoagulants), no EDTA or heparin plasma!

Stability of specimens
For longer transport send cooled at +2 to +8 °C, deep freezing is possible.

Sample Preparation

  • Place 50 µl reaction mix* into each well of a well filter plate.
  • Add 100 µl serum.
  • Agitate the filter plate for 60 s.
  • Incubate 30 min at +2 to +8°C.
  • Separate by centrifugation (1 min 1000 x g) or vacuum filtration (2 min, 300 mbar).
  • Add 200 µl water to each well of the collection plate.
  • Agitate the filter plate for 20 s.
  • Seal the collection plate and transfer to the autosampler.
  • Inject 150–200 µl of this mixture into the HPLC system.

* Preparation of reaction mix:
The reaction mix is prepared from Neutralisation Buffer, Stabilisation Buffer, Precipitation Reagent 1 and 2 (12.5 µl per sample).

Sample Stability

2 days at ambient temperature

Injection Volume

150-200 µl

Additional Info

For the Chromsystems HPLC analysis of CDT in serum with fast elution chromatography a ternary HPLC gradient system with UV detection is necessary.

Wavelength

460 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Controls

Controls available separately

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