54930-500 HPLC kit OneStep CDT serum fast elution

One Step CDT in Serum with Fast Elution - HPLC

Order no.: 54930/500
500 Tests
  • Asialotransferrin
  • Disialotransferrin
  • Pentasialotransferrin
  • Tetrasialotransferrin
  • Trisialotransferrin

With ready-to-use Pre-mixed Tubes
Only one pipetting step
Detects genetic variants


Clinical relevance

CDT (carbohydrate-deficient transferrin) is a lab marker showing the highest specificity for chronic alcohol abuse. CDT values increase significantly with the daily intake of more than 60 g ethanol (the equivalent of 0.75 l wine) per day over a period of two weeks. The values return to normal within two weeks in case of alcohol abstention. This makes CDT an excellent parameter for continuous monitoring in withdrawal programmes or for assessments in forensic or occupational medicine.

Unlike other biochemical parameters like γ-GT or MCV, liver diseases will not cause any false-positive results.


Product advantages

  • With ready-to-use Pre-mixed Tubes
  • Only one pipetting step required
  • Detection of genetic variants


This Chromsystems kit allows for the fast and reliable measurement of CDT in serum using HPLC and UV detection. Sample preparation with premixed tubes is performed in a single pipetting step, making it fast and convenient.

The CDT is displayed as an area percentage of total transferrin, so it remains unaffected by fluctuations in the concentration of transferrin in serum. Optimised chromatographic separation of transferrin isoforms also allows for the reliable detection of known genetic variants.

A manual method with automated sample preparation is also available as an alternative for greater sample throughput.


Technical Data

Method of Analysis


Number of Tests



Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin

Analysis Time

10 min

Limit of quantification

approx. 0.5 % Disialotransferrin


CV = 2.4 % (with 3.3 % Disialo)


CV = 3.2 % (with 3.3 % Disialo)


Serum (no anticoagulants, no EDTA or heparin plasma)

Pre-analytic Treatment

Time of sample drawing has no influence on the analysis, fasting serum preferred. Samples should be cooled during transport. Storage life is up to 1 week at +2 to +8 °C. For longer storage, deep-freeze below -18 °C.

Sample Preparation

  • Add 100 µl sample/control to a Pre-mixed Tube and mix 30-60 s (vortex).
  • Incubate 30 min at +2 to +8°C.
  • Centrifuge 10 min at 9000 x g.
  • Inject 150–200 µl of the undiluted supernatant into the HPLC system.

Sample Stability

6 days at ambient temperature

Additional Info

For the Chromsystems HPLC analysis of One Step CDT in serum (Fast Elution) any ternary HPLC gradient system with UV detection is suitable.

Injection Volume

150-200 µl

Flow rate

Detection: 1.5 ml/min, Rinsing: 2.5 ml/min

Column temperature

ambient (~25 °C)


460 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase A

Order no.: 54931

Mobile Phase B

Order no.: 54932

Mobile Phase C

Order no.: 54933

Pre-mixed Tubes

Order no.: 54934


Controls available separately