26000 HPLC kit olanzapine desmethylolanzapine serum plasma

Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC

Order no.: 26000
100 Tests
  • N-Desmethylolanzapine
  • Olanzapine
  • Quetiapine


Low limit of quantification
Robust UV-VIS detection
Metabolite included

Description

Clinical relevance

Olanzapine and quetiapine are highly effective antipsychotic agents used to treat schizophrenic psychosis. Like clozapine, they are both atypical neuroleptics which are characterised by low levels of side effects in the extrapyramidal motor system (dyskinesia, Parkinson’s disease). Quetiapine is structurally related to olanzapine and is approved as a neuroleptic, but it shows a different receptor profile.

It is essential that the serum levels of these psychotropic drugs be monitored to ensure an optimal therapeutic effect and to minimise any undesirable side effects resulting from overdose. Furthermore, patient compliance gets checked and confirmed by this procedure.

 

Product advantages

  • Low limit of quantification
  • Robust UV-VIS detection
  • Metabolite analysis included

 

This kit can be used to reliably determine the levels of olanzapine, its metabolite desmethylolanzapine, and quetiapine in serum or plasma. The sample preparation is based on an efficient and selective solid phase extraction including an internal standard. The chromatographic separation is run on an isocratic HPLC system with UV-VIS detector. When used with additional specific calibrators and controls, perazine and citalopram can also be determined in serum or plasma with this kit.

For routine diagnostic tests of these analytes by LC-MS/MS, our MassTox® Series A product with the respective parameter sets is available.

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

100

Parameter

N-Desmethylolanzapine, Olanzapine, Quetiapine

Analysis Time

< 6 min; (Quetiapine approx. 18 min)

Limit of quantification

Olanzapine/Desmethylolanzapine: approx. 4 ng/ml* (therapeutical range: 20-80 ng/ml)

Quetiapine: approx. 11 ng/ml*: (therapeutical range: 40-400 ng/ml)

*depending on the employed detector

Linearity

Olanzapine/Desmethylolanzapine: up to 1000 ng/ml
Quetiapine: up to 3000 ng/ml

Recovery

Olanzapine/Desmethylolanzapine: 95 %; Quetiapine: 97 %

Intraassay

Olanzapine/Desmethylolanzapine: CV < 5 %
Quetiapine: CV < 4 %

Interassay

Olanzapine/Desmethylolanzapine: CV < 6 %
Quetiapine: CV < 4 %

Column temperature

ambient (~25 °C)

Flow rate

0.6 ml/min

Specimen

Serum/Plasma

Sample Preparation

Extraction

  • Equilibrate the sample clean up columns with 1 ml Equilibration Buffer 1 following by 1 ml Equilibration Buffer 2.
  • Apply 1 ml sample and then 100 µl Internal Standard to the sample clean up column, draw through completely by centrifugation or suction, discard effluent.
  • Apply 1 ml Wash Buffer, draw through by centrifugaton or suction, discard effluent, repeat once.
  • Elute with 400 µl Elution Buffer.
  • Inject 50 µl eluate into the HPLC system.

Sample Stability

At +2 to +8 °C up to 1 week**. Keep specimens cool for transport and freeze for longer storage. (**Dusci et al., (2002) J Chromatography B 773(2): 191-197)

Injection Volume

50 µl

Additional Info

For the HPLC analysis of olanzapine in serum/plasma any isocratic HLPC system with UV-VIS detector is suitable.

Wavelength

254 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Calibrators

Controls