26000 HPLC kit olanzapine desmethylolanzapine serum plasma

Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC

Order no.: 26000
100 Tests
  • N-Desmethylolanzapine
  • Olanzapine
  • Quetiapine

Low limit of quantification
Robust UV-VIS detection
Metabolite included


Clinical relevance

Olanzapine and quetiapine are highly effective antipsychotic agents used to treat schizophrenic psychosis. Like clozapine, they are both atypical neuroleptics which are characterised by low levels of side effects in the extrapyramidal motor system (dyskinesia, Parkinson’s disease). Quetiapine is structurally related to olanzapine and is approved as a neuroleptic, but it shows a different receptor profile.

It is essential that the serum levels of these psychotropic drugs be monitored to ensure an optimal therapeutic effect and to minimise any undesirable side effects resulting from overdose. Furthermore, patient compliance gets checked and confirmed by this procedure.


Product advantages

  • Low limit of quantification
  • Robust UV-VIS detection
  • Metabolite analysis included


This kit can be used to reliably determine the levels of olanzapine, its metabolite desmethylolanzapine, and quetiapine in serum or plasma. The sample preparation is based on an efficient and selective solid phase extraction including an internal standard. The chromatographic separation is run on an isocratic HPLC system with UV-VIS detector. When used with additional specific calibrators and controls, perazine and citalopram can also be determined in serum or plasma with this kit.

For routine diagnostic tests of these analytes by LC-MS/MS, our MassTox® Series A product with the respective parameter sets is available.


Technical Data

Method of Analysis


Number of Tests



N-Desmethylolanzapine, Olanzapine, Quetiapine

Analysis Time

18 min

Limit of quantification

Olanzapine/Desmethylolanzapine: approx. 3.5 ng/ml

Quetiapine: approx. 11 ng/ml


Olanzapine/Desmethylolanzapine: up to 1000 ng/ml
Quetiapine: up to 5000 ng/ml


Olanzapine/Desmethylolanzapine: 95 %; Quetiapine: 96 %


Olanzapine/Desmethylolanzapine: CV < 4.8 %
Quetiapine: CV < 4.1 %


Olanzapine/Desmethylolanzapine: CV < 9.6 %
Quetiapine: CV < 7.2 %



Pre-analytic Treatment

Patient samples are stable cooled (+4 °C) in the dark for at least 24 h. For longer storage keep samples at approx. -20 °C.

Sample Preparation


  • Equilibrate the sample clean up columns with 1 ml Equilibration Buffer 1 following by 1 ml Equilibration Buffer 2.
  • Apply 1 ml sample and then 100 µl Internal Standard to the sample clean up column, draw through completely by centrifugation or suction, discard effluent.
  • Apply 1 ml Wash Buffer, draw through by centrifugaton or suction, discard effluent, repeat once.
  • Elute with 400 µl Elution Buffer.
  • Inject 50 µl eluate into the HPLC system.

Sample Stability

In glass vials and protected from light, the prepared samples are stable at room temperature (+18 to +30 °C) or refrigerated (+2 to +8 °C) for at least 14 days. Below -18 °C only quetiapin is stable.

Additional Info

Any isocratic HLPC system with UV-VIS detector is suitable.

Injection Volume

50 µl

Flow rate

0.6 ml/min



Column temperature

ambient (~ 25 °C)


254 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately