46000 HPLC kit mycophenolic acid plasma serum

Mycophenolic Acid in Plasma/Serum - HPLC

Order no.: 46000
100 Tests
  • Mycophenolic acid

Fast and easy sample preparation
Removes interfering substanzes for a precise analysis
Detection of physiologically active MPA

 

Description

Clinical relevance

Mycophenolic acid (MPA) is used as an immunosuppressant agent to prevent organ transplant rejection. It is also used to treat autoimmune disorders with chronic inflammation of the skin (psoriasis), eyes, and the digestive tract (Crohn's disease).

Currently available drugs contain mycophenolate mofetil or mycophenolate sodium, which are both completely metabolised into the active metabolite MPA. MPA is partially further metabolised into the inactive mycophenolate glucuronide (MPAG), which then can undergo deglucuronidation in the enterohepatic circulation system and is available again in the body as an active metabolite. Therapeutic monitoring of MPA is required to reach blood concentrations within the therapeutic window to achieve a favourable ratio between therapeutic effects and side effects for the patient.

 

Product advantages

  • Simple and fast sample preparation
  • No interferences

 

This Chromsystems kit is designed to determine the active metabolite mycophenolic acid in plasma or serum using HPLC. Sample preparation is performed by means of selective solid phase extraction, in which physiologically inactive MPAG is reliably and clearly separated. A stable internal standard ensures exact quantification. The HPLC column separates interfering substances, including the antibiotic daptomycin, to guarantee reliable and reproducible results.

A separate kit (# 92916) is available for the LC-MS/MS analysis of MPA and MPAG.

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

100

Parameter

Mycophenolic acid

Analysis Time

8-9 min

Limit of quantification

0.1 mg/l

Linearity

up to 30 mg/l

Recovery

> 97 %

Intraassay

CV = 1.7-2-0 %

Interassay

CV = 2.1-2.8 %

Gradient

Isocratic

Column temperature

ambient (~25 °C)

Flow rate

1 ml/min

Specimen

plasma/serum

Sample Preparation

  • Centrifuge specimens before sample preparation (10 min at 2800 x g)!
  • Precondition the Sample Clean Up Columns with 1 ml Equilibration Buffer 1 and draw through by centrifugation (approx. 30–60 s at 190 x g) or suction.
  • Repeat with 1 ml Equilibration Buffer 2.
  • Mix 250 μl plasma/serum sample with 250 μl Internal Standard.
  • Apply complete sample to the Sample Clean Up Column and draw through by centrifugation (1 min at 190 x g) or suction; discard effluent.
  • Draw 2 x 1 ml Wash Buffer through Sample Clean Up Column by centrifugation (2 min at 1100 x g) or suction; discard effluent.
  • Elute the mycophenolic acid with 400 μl of the Elution Buffer.
  • Inject 20 - 40 μl eluate into the HPLC system.

Sample Stability

Specimens should be kept cool for transport. Storage life is up to 5 days at +2 to +8 °C. For longer storage, deep-freeze below –18 °C.

Injection Volume

20 - 40 µl

Flow

1 ml/min

Additional Info

For the analysis of mycophenolic acid any isocratic HPLC system with UV detector is suitable.

Wavelength

215 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Calibrators

Controls

Controls available separately

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