MassChrom® Amino Acids and Acylcarnitines from Dried Blood / Non Derivatised - LC-MS/MS

Order No.: 57000/F, for 960 tests
Parameters:
Acylcarnitines & free Carnitine, Amino Acids

Sample prep without derivatisation of the analytes
With 96 well filter plates

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

Amino Acids and Succinylacetone Acylcarnitines and free Carnitine
Alanine Free Carnitine
Arginine C2-Carnitine
Aspartic acid C3-Carnitine
Citrulline C4-Carnitine
Glutamic acid C5-Carnitine
Glycine C5DC-Carnitine
Leucine C6-Carnitine
Methionine C8-Carnitine
Ornithine C10-Carnitine
Phenylalanine C12-Carnitine
Proline C14-Carnitine
Tyrosine C16-Carnitine
Valine C18-Carnitine
Succinylacetone (included in the Upgrade Set 57111/F)  

Clinical relevance

Newborn screening aims to detect congenital metabolic disorders early on before they can cause harm to the organism. Included in these diseases are defects of amino acid metabolism such as phenylketonuria (PKU) and maple syrup urine disease (MSUD), defects of fatty acid metabolism and organic acidurias.

Mass spectrometry is the method of choice for diagnostic testing: it enables to check for a broad range of metabolic defects in a single run. An HPLC column is not required due to the high selectivity of tandem mass spectrometry.

 

Product advantages

  • Simple SUAC determination with upgrade set
  • Screening for many metabolic disorders in a single run such as PKU (Phenylketonuria), MSUD (Maple Syrup Urine Disease) and tyrosinemia type I
  • Each analyte safeguarded by its own internal standard

 

Assay characteristics

This Chromsystems assay allows the fast determination of amino acids and acylcarnitines from dried blood spot samples as part of the newborn screening for amino acid and fatty acid metabolic disorders using tandem mass spectrometry. Sample preparation is based on effective extraction of the analytes from the blood spot without any further sample preparation steps required.

Detailed performance evaluation data for this assay can be found in Appendix III of the instruction manual.

 

For the analysis of succinylacetone an upgrade set is available (order no. 57111/F).

Here's an overview of all our newborn screening assays.

More Information
Method of Analysis FIA-MS/MS
Number of Tests 960
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. Detailed performance evaluation data for this assay can be found in Appendix III of the instruction manual.
Lower and Upper Limit of Quantitation

LLOQ:
Amino acids: 0.46-26.9 µmol/l
Free Carnitine: 0.367 µmol/l
Acylcarnitines: 0.008-0.166 µmol/l

SUAC: 0.478 µmol/l (only with upgrade set)

ULOQ:
Amino acids: 2500-5000 µmol/l
Free Carnitine: 300 µmol/l
Acylcarnitines: 22.5-400 µmol/l

SUAC: 120 µmol/l (only with upgrade set)

For the values of all analytes in detail please refer to the instruction manual.

Different systems might show different performance data.

Specimen Dried blood
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Punch out 3.2 mm blood spots from the filter cards into a 96 Well Filter Plate.
  • Add 200 µl Internal Standard and agitate for 20 min.
  • Draw through supernatant into the 96 Well Plate by centrifugation for 5 min.
  • Inject up to 10 µl into the LC-MS/MS system.

In the sample preparation for the analysis of succinylacetone the following additional steps have to be performed before injection:
Add Internal Standard and Extraction Buffer, agitate 30 min, combine extracts by centrifuging, incubate 45 min.

For details please refer to the instruction manual.

Run Time < 2 min
Injection Volume <= 10 µl
Flow Rate 20–600 µl/min
MS/MS Mode MRM
Additional Info

No HPLC column is required.

Information

Mass transitions of analytes and internal standards

Amino acids and SUAC
MRM
Alanine 90 > 44
Alanine-D4 94 > 48
Arginine 175 > 70
Arginine-D7 182 > 77
Aspartic acid 134 > 116
Aspartic acid-D3 137 > 119
Citrulline 176 > 113
Citrulline-D2 178 > 115
Glutamic acid 148 > 130
Glutamic acid-D5 153 > 135
Glycine 76 > 30
Glycine-13C2-15N 79 > 32
Leucine 132 > 86
Leucine-D3 135 > 89
Methionine 150 > 133
Methionine-D3 153 > 136
Ornithine 133 > 70
Ornithine-D6 139 > 76
Phenylalanine 166 > 120
Phenylalanine-D5 171 > 125
Proline 116 > 70
Proline-D7 123 > 77
Tyrosine 182 > 136
Tyrosine-D4 186 > 140
Valine 118 > 72
Valine-D8 126 > 80
SUAC 155 > 137
SUAC-13C5 160 > 142
Acylcarnitines and free carnitine MRM
Carnitine 162 > 85
Carnitine-D9 171 > 85
C2-Carnitine 204 > 85
C2-Carnitine-D3 207 > 85
C3-Carnitine 218 > 85
C3-Carnitine-D3 221 > 85
C4-Carnitine 232 > 85
C4-Carnitine-D3 235 > 85
C5-Carnitine 246 > 85
C5-Carnitine-D9 255 > 85
C5DC-Carnitine 276 > 85
C5DC-Carnitine-D6 282 > 85
C6-Carnitine 260 > 85
C6-Carnitine-D3 263 > 85
C8-Carnitine 288 > 85
C8-Carnitine-D3 291 > 85
C10-Carnitine 316 > 85
C10-Carnitine-D3 319 > 85
C12-Carnitine 344 > 85
C12-Carnitine-D3 347 > 85
C14-Carnitine 428 > 85
C14-Carnitine-D3 375 > 85
C16-Carnitine 400 > 85
C16-Carnitine-D3 403 > 85
C18-Carnitine 428 > 85
C18-Carnitine-D3 431 > 85
Parameters Acylcarnitines & free Carnitine, Amino Acids
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.
Amino Acids and Succinylacetone Acylcarnitines and free Carnitine
Alanine Free Carnitine
Arginine C2-Carnitine
Aspartic acid C3-Carnitine
Citrulline C4-Carnitine
Glutamic acid C5-Carnitine
Glycine C5DC-Carnitine
Leucine C6-Carnitine
Methionine C8-Carnitine
Ornithine C10-Carnitine
Phenylalanine C12-Carnitine
Proline C14-Carnitine
Tyrosine C16-Carnitine
Valine C18-Carnitine
Succinylacetone (included in the Upgrade Set 57111/F)  

Clinical relevance

Newborn screening aims to detect congenital metabolic disorders early on before they can cause harm to the organism. Included in these diseases are defects of amino acid metabolism such as phenylketonuria (PKU) and maple syrup urine disease (MSUD), defects of fatty acid metabolism and organic acidurias.

Mass spectrometry is the method of choice for diagnostic testing: it enables to check for a broad range of metabolic defects in a single run. An HPLC column is not required due to the high selectivity of tandem mass spectrometry.

 

Product advantages

  • Simple SUAC determination with upgrade set
  • Screening for many metabolic disorders in a single run such as PKU (Phenylketonuria), MSUD (Maple Syrup Urine Disease) and tyrosinemia type I
  • Each analyte safeguarded by its own internal standard

 

Assay characteristics

This Chromsystems assay allows the fast determination of amino acids and acylcarnitines from dried blood spot samples as part of the newborn screening for amino acid and fatty acid metabolic disorders using tandem mass spectrometry. Sample preparation is based on effective extraction of the analytes from the blood spot without any further sample preparation steps required.

Detailed performance evaluation data for this assay can be found in Appendix III of the instruction manual.

 

For the analysis of succinylacetone an upgrade set is available (order no. 57111/F).

Here's an overview of all our newborn screening assays.

More Information
Method of Analysis FIA-MS/MS
Number of Tests 960
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. Detailed performance evaluation data for this assay can be found in Appendix III of the instruction manual.
Lower and Upper Limit of Quantitation

LLOQ:
Amino acids: 0.46-26.9 µmol/l
Free Carnitine: 0.367 µmol/l
Acylcarnitines: 0.008-0.166 µmol/l

SUAC: 0.478 µmol/l (only with upgrade set)

ULOQ:
Amino acids: 2500-5000 µmol/l
Free Carnitine: 300 µmol/l
Acylcarnitines: 22.5-400 µmol/l

SUAC: 120 µmol/l (only with upgrade set)

For the values of all analytes in detail please refer to the instruction manual.

Different systems might show different performance data.

Specimen Dried blood
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Punch out 3.2 mm blood spots from the filter cards into a 96 Well Filter Plate.
  • Add 200 µl Internal Standard and agitate for 20 min.
  • Draw through supernatant into the 96 Well Plate by centrifugation for 5 min.
  • Inject up to 10 µl into the LC-MS/MS system.

In the sample preparation for the analysis of succinylacetone the following additional steps have to be performed before injection:
Add Internal Standard and Extraction Buffer, agitate 30 min, combine extracts by centrifuging, incubate 45 min.

For details please refer to the instruction manual.

Run Time < 2 min
Injection Volume <= 10 µl
Flow Rate 20–600 µl/min
MS/MS Mode MRM
Additional Info

No HPLC column is required.

Information

Mass transitions of analytes and internal standards

Amino acids and SUAC
MRM
Alanine 90 > 44
Alanine-D4 94 > 48
Arginine 175 > 70
Arginine-D7 182 > 77
Aspartic acid 134 > 116
Aspartic acid-D3 137 > 119
Citrulline 176 > 113
Citrulline-D2 178 > 115
Glutamic acid 148 > 130
Glutamic acid-D5 153 > 135
Glycine 76 > 30
Glycine-13C2-15N 79 > 32
Leucine 132 > 86
Leucine-D3 135 > 89
Methionine 150 > 133
Methionine-D3 153 > 136
Ornithine 133 > 70
Ornithine-D6 139 > 76
Phenylalanine 166 > 120
Phenylalanine-D5 171 > 125
Proline 116 > 70
Proline-D7 123 > 77
Tyrosine 182 > 136
Tyrosine-D4 186 > 140
Valine 118 > 72
Valine-D8 126 > 80
SUAC 155 > 137
SUAC-13C5 160 > 142
Acylcarnitines and free carnitine MRM
Carnitine 162 > 85
Carnitine-D9 171 > 85
C2-Carnitine 204 > 85
C2-Carnitine-D3 207 > 85
C3-Carnitine 218 > 85
C3-Carnitine-D3 221 > 85
C4-Carnitine 232 > 85
C4-Carnitine-D3 235 > 85
C5-Carnitine 246 > 85
C5-Carnitine-D9 255 > 85
C5DC-Carnitine 276 > 85
C5DC-Carnitine-D6 282 > 85
C6-Carnitine 260 > 85
C6-Carnitine-D3 263 > 85
C8-Carnitine 288 > 85
C8-Carnitine-D3 291 > 85
C10-Carnitine 316 > 85
C10-Carnitine-D3 319 > 85
C12-Carnitine 344 > 85
C12-Carnitine-D3 347 > 85
C14-Carnitine 428 > 85
C14-Carnitine-D3 375 > 85
C16-Carnitine 400 > 85
C16-Carnitine-D3 403 > 85
C18-Carnitine 428 > 85
C18-Carnitine-D3 431 > 85
Parameters Acylcarnitines & free Carnitine, Amino Acids
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.