MassChrom® Cortisol, Cortisone in Saliva - LC-MS/MS

MassChrom® Cortisol, Cortisone in Saliva - LC-MS/MS

Order no.: 73000
400 Tests
  • Cortisol
  • Cortisone

Simple and non-invasive sample collection
Easy sample preparation
Complete solution from sample to result
Reliable, robust and proven CE-IVD method

Description

Clinical relevance

Cortisol and cortisone are steroid hormones that play a role in a range of diseases - one of the most common uses in clinical laboratories is the screening of Cushing’s syndrome and Addison’s disease. However, symptoms are not specific, requiring the precise and efficient determination of these parameters in the clinical laboratory.

 

Product advantages

  • Simple and non-invasive sample collection
    Saliva is a matrix that can be obtained very easily and for patients in a non-invasive manner. Stress-induced adrenal stress responses are thereby eliminated, making salivary sampling more reliable. This is of particular importance in stress research and pediatric applications.
  • Easy sample preparation
    The efficient sample preparation takes place in just a few steps.
  • Complete solution from sample to result
    This assay offers everything required for the determination, eliminating the need to develop your own laboratory tests. We also provide you with an excellent on-site support for installation, testing and more.
  • Reliable, robust and proven CE-IVD method
    With our CE-IVD method you gain access to LC-MS/MS as the best available assay for an accurate steroid analysis. The parameters are determined directly without the risk of cross-reactivity as described for immunoassays. You can trust your test results with specific co-eluting internal standards, human-matrix based multilevel calibrators and MassCheck® controls.

 

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Number of Tests

400

Parameter

Cortisol, Cortisone

Analysis Time

4.6 min

Limit of quantification

0.3 µg/l

Linearity

up to 120 μg/l (Cortisol)
up to 180 μg/l (Cortisone)

Recovery

96–105 %

Intraassay

CV = 2–5 %

Interassay

CV = 2–7 %

Gradient

binary

Column temperature

ambient (~ 25 °C)

Specimen

Saliva

Sample Preparation

  • Pipette 100 μl sample/calibrator/MassCheck® control into a Clean-Up Tube.
  • Add 50 μl Internal Standard Mix.
  • Centrifuge 15 min at 14000 x g.
  • Transfer each filtrate into a 1.5 ml vial equipped with micro-inserts.
  • Seal each vial and transfer into autosampler.
  • Inject up to 30 μl.

Injection Volume

≤ 30 µl

Ionisation

ESI positive

MS/MS-Mode

MRM

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase A


Order no.: 73001

Mobile Phase B


Order no.: 73002

Internal Standard Mix


Order no.: 73004

Clean-Up Tubes


Order no.: 73008

Rinsing Solution


Order no.: 73009

Calibrators

Calibrators available separately

Controls

Controls available separately

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