75111 Amino Acid Analysis in Plasma/Serum with Reaction Vials

MassChrom® Amino Acid Analysis in Plasma/Serum with Reaction Vials

Order no.: 75111
3 x 96 Tests

Quantitative Amino Acid Analysis (AAA) of 48 amino acids
Full test menu in less than 20 min or PKU/MSUD panel in 8-9 min
Sample prep with reaction vials

Description

Clinical relevance

Metabolic disorders are caused by the failure of specific metabolic pathways in the human body. They are often based on hereditary enzyme defects, which leads to a decrease or suppression of enzyme activity. Subsequently, this can result in the accumulation of potentially toxic metabolites, which can cause organ defects and multi-systemic diseases.

The identification of metabolic diseases is routinely performed by newborn screening. Once identified, life-long, specific, low-protein diet can prevent symptoms from occurring. Continuous monitoring of the plasma concentrations of the metabolic products is required to monitor and adjust the patient‘s dietary prescription. Furthermore, an amino acid analysis can also be performed for patients with unclear clinical signs.

 

Product advantages

  • Quantitative Amino Acid Analysis (AAA) of 48 analytes
  • No derivatisation
  • Short run times
  • Excellent separation of isobaric analytes
  • Each analyte safeguarded by its own internal standard
  • Full test menu in less than 20 min or PKU/MSUD panel in 8-9 min

 

This assay is designed for the quantitative determination of amino acids in plasma/serum. It can be used for a range of applications including follow-up testing in newborn screening, therapy monitoring as well as for other amino acid testing purposes, such as aminoacidopathy diagnosis or nutritional research. The kit covers the 48 most important amino acids that are analysed within 20 min. Alternatively, the same assay allows the determination of 7 parameters for the common metabolic diseases PKU and MSUD in less than 8 min. The method runs on the same chromatographic setup and includes a shorter gradient. The sample prep consists of a very fast and simple protein precipitation, eliminating the need of time consuming and error-prone derivatisation steps. The use of stable isotope-labelled, co-eluting internal standards, and the multilevel calibrators ensures reproducible and dependable quantification of the analytes. The assay is available in 2 versions either with reaction vials or with 96 deep well plates.

 

The following 48 amino acids can be measured quantitatively with the assay MassChrom® Amino Acid Analysis in Plasma/Serum

Acetyltyrosine
Adenosylhomocysteine
Alanine
β-Alanine
α-Aminoadipic acid
α-Aminobutyric acid
β-Aminoisobutyric acid
γ-Aminobutyric acid
Anserine
Arginine
Argininosuccinic acid
Asparagine
Aspartic acid
Carnosine
Citrulline
Homocitrulline
Cystathionine
Cystine
Cysteine S-sulfate
Homocystine
Ethanolamine
Phosphoethanolamine
Glutamic acid
Glutamine
Glycine
Histidine
1-Methylhistidine
3-Methylhistidine
Leucine
Isoleucine
Allo-Isoleucine
Lysine
Hydroxylysine
Methionine
Ornithine
Phenylalanine
Phosphoserine
Pipecolic acid
Proline
4-Hydroxyproline
Saccharopine
Sarcosine
Serine
Taurine
Threonine
Tryptophan
Tyrosine
Valine

The 7 analytes highlighted in red can be measured with both the full panel and the PKU/MSUD panel.

 

Chromatogram

Technical Data

Method of Analysis

LC-MS/MS

Number of Tests

3 x 96

Analysis Time

< 20 min (Full Panel), 8-9 min (PKU/MSUD Panel)

Limit of quantification

down to 0.1 μmol/l

Linearity

up to 3-fold concentration of highest calibrator

Intraassay

CV < 13 % (Full Panel),
CV < 6 % (PKU/MSUD Panel)

Interassay

CV < 10 % (Full Panel),
CV < 7 % (PKU/MSUD Panel)

Specimen

Plasma/Serum

Sample Preparation

  • Pipette 50 μl reconstituted Internal Standard Mix
    + 25 μl sample/calibrator/MassCheck® control
    + 400 μl Precipitation Reagent into a reaction vial.
  • Mix for 30 s (vortex) and centrifuge for 5 min at 16000 x g.
  • Transfer supernatant into autosampler vial.
  • Inject 2–5 μl into the LC-MS/MS system.

Injection Volume

2–5 μl

Column temperature

ambient (~ 25 °C)

Ionisation

ESI positive

MS/MS-Mode

MRM

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Calibrators

Calibrators available separately

Controls

Controls available separately