24000 HPLC kit levetiracetam (Keppra) serum plasma

Levetiracetam (Keppra®) in Serum/Plasma - HPLC

Order no.: 24000
100 Tests
  • Levetiracetam (Keppra®)


Completes the analysis of antiepileptic drugs
Efficient removal of interferences
High sample throughput

Description

Clinical relevance

Levetiracetam (Keppra®) is an antiepileptic that bears no structural similarity to other drugs. In the cells of the brain, Levetiracetam acts to provide presynaptic inhibition over multiple cascades of postsynaptic chloride ion influx.

It is approved for monotherapy of certain forms of epilepsy in patients of age 16 years and older. It may also be prescribed as add-on therapy for younger patients, e.g. for juvenile myoclonic epilepsy (also called Janz syndrome). The determination of the serum concentration of levetiracetam is important since it undergoes individual fluctuations which vary from patient to patient.

 

Product advantages

  • Completion of the antiepileptic analysis
  • Efficient removal of interferences
  • Exact quantification with a tailored internal standard

 

This kit allows for the rapid and reliable determination of levetiracetam levels in serum or plasma. Sample preparation is based on an efficient and fast method of solid phase extraction (SPE). Interference from other antiepileptics or their metabolites is prevented. A tailored internal standard is used to achieve an exact quantification. This kit enables isocratic elution for chromatographic separation on an RP column with subsequent UV detection.

Another HPLC kit (# 22000) that can determine further antiepileptics in serum/plasma is also available. Additionally a parameter set is available that uses mass spectrometry for analysis (MassTox® TDM Series A) which can measure 26 antiepileptics.

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

100

Parameter

Levetiracetam (Keppra®)

Analysis Time

< 5 min

Limit of quantification

0.5 mg/l

Linearity

up to 1000 mg/l

Recovery

90 %

Intraassay

CV < 3 %

Interassay

CV < 5 %

Column temperature

ambient (~25 °C)

Flow rate

1.5 ml/min

Specimen

Serum/Plasma

Pre-analytic Treatment

 

 

Sample Preparation

Extraktion:

  • Condition the sample clean up columns with 1 ml Equilibration Buffer 1 and 1 ml Equilibration Buffer 2 (centrifugation 1 min at 2200 rpm).
  • Apply 100 µl serum/plasma followed by 100 µl Internal Standard to the sample clean up column, mix.
  • Draw through completely by centrifugation (3 min at 3000 rpm), discard effluent.

Wash:

  • Apply 1 ml of Wash Buffer 1 , centrifuge 1 min at 3000 rpm, discard effluent. Repeat with 1 ml of Wash Buffer 2.

Elution:

  • Elute Levetiracetam with 500 µl Elution Buffer (1 min at 2200 rpm).
  • Inject 10 µl eluate into the HPLC system.

Sample Stability

At +2 to +8 °C up to 1 week. Keep specimens cool for transport and freeze for longer storage.

Injection Volume

10 µl

Additional Info

For the HPLC analysis of Levetiracetam (Keppra®) any isocratic HPLC system with UV detector is suitable.

Wavelength

210 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase


Order no.: 24001

Internal Standard


Order no.: 24004

Equilibration Buffer 1


Order no.: 24005

Equilibration Buffer 2


Order no.: 24006

Wash Buffer 1


Order no.: 24007

Wash Buffer 2


Order no.: 24009

Elution Buffer


Order no.: 24010

Sample Clean Up Columns


Order no.: 24008

Calibrators

Calibrators available separately

Controls

Controls available separately

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