27037 HPLC kit itraconazole posaconazole voriconazole serum plasma

Itraconazole, Posaconazole and Voriconazole in Serum/Plasma - HPLC

Order no.: 27037
100 Tests
  • Hydroxyitraconazole
  • Itraconazole
  • Posaconazole
  • Voriconazole

Itraconazole/posaconazole analysis upgraded with voriconazole
Tri-level controls available
Custom-made internal standard

Description

Clinical relevance

In recent years there has been an increase in invasive fungal infections due to the growing number of people with immunosuppression. Candida and Aspergillus are the most common types of infection, but previously rare fungal infections such as fusariosis or zygomycosis are also on the rise. Options for oral antimycotic therapy have improved considerably with the advent of new drugs. These include second-generation triazoles such as posaconazole and voriconazole.

Posaconazole is used primarily in case of therapy-resistant mycoses or drug intolerances. Voriconazole is also effective against some itraconazole-resistant Aspergillus species and offers fungicidal activity against emerging fungal pathogens such as Scedosporium or Fusarium. All three azoles exhibit wide inter-individual variability in bioavailability, so monitoring of drug levels is required.

 

Product advantages

  • 3PLUS1® Multilevel Calibrator Set available
  • Tri-level controls available
  • Tailor-made internal standard

 

This Chromsystems kit allows for reliable chromatographic determination of itraconazole, its active metabolite hydroxy-itraconazole, and posaconazole and voriconazole in only one isocratic HPLC run with fluorescence detection. Two very simple and efficient protein precipitation steps are used to separate interfering components and to obtain stable eluates. A tailor-made internal standard ensures reliable and precise quantification of the analytes.

These analytes - together with four further antimycotics - can also be determined using LC-MS/MS with the Parameter Set for Antimycotic Drugs from the MassTox® Series A.

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

100

Parameter

Hydroxyitraconazole, Itraconazole, Posaconazole, Voriconazole

Analysis Time

Itraconazole/OH-Itraconazole: approx. 16 min; Posaconazole/Voriconazole: approx. 9 min

Limit of quantification

Itraconazole/OH-Itraconazole: 0.03 mg/l
Posaconazole 0.02 mg/l
Voriconazole: 0.2 mg/l

Linearity

Itraconazole/OH-Itraconazole: up to 10 mg/l
Posaconazole: up to 10 mg/l
Voriconazole: up to 20 mg/l

Recovery

> 98 %

Intraassay

CV < 4.4 %

Interassay

CV < 5.2 %

Information

Stability of prepared samples: at least 7 days at ambient temperature

Column temperature

+25 °C

Flow rate

1.2 ml/min

Specimen

Serum/Plasma

Sample Preparation

  • Mix 100 µl serum/plasma, 25 µl Internal Standard and 25 µl Precipitation Reagent 1 in a reaction vial and vortex briefly.
  • Add 200 µl Precipitation Reagent 2 and vortex 30 s.
  • Centrifuge 5 min at 15000 x g
  • Inject 20 µl of the supernatant into the HPLC system..

Sample Stability

At ambient temperature or cooled (+2 to +8 °C) for at least 7 days. Deep-freeze for longer storage.

Injection Volume

10 to 20 µl

Additional Info

For the HPLC analysis of itraconazole, posaconazole and voriconazole any isocratic HPLC system with fluorescence detector is suitable.

Wavelength

EX 261 nm

EM 366 nm

Autosampler rinsing solution

80 % water
20 % acetonitril + 0.2 % phosphoric acid (85 per cent)

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Calibrators

Controls

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