Internal Standard

Internal Standard

Order no.: 57004

MassChrom® Amino Acids and Acylcarnitines from Dried Blood/Non Derivatised - LC-MS/MS

Description

Lyophilised internal standard amino acids, acylcarnitines.

Technical Data

Content

4 x 25 ml (lyoph.)

Concentrations

Please note: All concentrations shown here are exemplatory. They do not reflect real data for the described products. Actual concentrations depend on the production lot of each product and can exclusively be found in the product information leaflet. Please ask for these product information leaflets or download them from the service site of the Chromsystems website.

SubstanceUnitConcentrationConcentration in sample
(100 µl IS/3.1 µl blood)
Alanine-D4 mg/l
µmol/l
1.12
12.02
36
388
Arginine-D7 mg/l
µmol/l
0.54
3.00
18
97
Aspartic acid-D3 mg/l
µmol/l
1.63
12.00
53
387
Citrulline-D2 mg/l
µmol/l
0.55
3.12
18
101
Glutamic acid-D5 mg/l
µmol/l
1.83
12.00
59
387
Glycine-13C2, 15N mg/l
µmol/l
1.67
21.45
54
692
Leucine-D3 mg/l
µmol/l
1.65
12.30
53
397
Methionine-D3 mg/l
µmol/l
0.71
4.68
23
151
Ornithine-D6 mg/l
µmol/l
1.01
7.32
33
236
Phenylalanine-D5 mg/l
µmol/l
1.05
6.18
34
199
Tyrosine-D4 mg/l
µmol/l
1.64
8.84
53
285
Valine-D8 mg/l
µmol/l
1.11
8.88
36
286
C0-Carnitine-D9 mg/l
µmol/l
0.131
0.771
4.24
24.88
C2-Carnitine-D3 mg/l
µmol/l
0.065
0.317
2.11
10.21
C3-Carnitine-D3 mg/l
µmol/l
0.018
0.084
0.59
2.70
C4-Carnitine-D3 mg/l
µmol/l
0.009
0.040
0.30
1.30
C5-Carnitine-D9 mg/l
µmol/l
0.011
0.045
0.37
1.46
C5DC-Carnitine-D6 mg/l
µmol/l
0.010
0.036
0.32
1.15
C6-Carnitine-D3 mg/l
µmol/l
0.010
0.039
0.33
1.26
C8-Carnitine-D3 mg/l
µmol/l
0.012
0.041
0.39
1.33
C10-Carnitine-D3 mg/l
µmol/l
0.014
0.044
0.45
1.42
C12-Carnitine-D3 mg/l
µmol/l
0.014
0.041
0.46
1.32
C14-Carnitine-D3 mg/l
µmol/l
0.014
0.037
0.45
1.21
C16-Carnitine-D3 mg/l
µmol/l
0.031
0.078
1.01
2.52
C18-Carnitine-D3 mg/l
µmol/l
0.030
0.070
0.97
2.25

Manual

During the determination of amino acids and acylcarnitines within the scope of newborn screening the internal standard is used as an individual calibrator for each sample, so matrix effects are reduced to a minimum.

The internal standard is added directly to the dried blood spot disk and is prepared and analysed together with the blood sample.

Reconstitution

Remove the seal and rubber stopper from the vial. Reconstitute the lyophilised internal standard with exactly 25 ml of extraction buffer (order no. 57008). First add 5 ml of extraction buffer into the vial. Replace the stopper and allow to sit for 5 to 10 min at room temperature. Swirl the vial to dissolve the contents until homogeneity.

Then transfer the content of the vial into a 25 ml volumetric flask. Wash the vial two times with 5 ml extraction buffer and combine the solutions. Dilute the volumetric flask with extraction buffer to exactly 50 ml and swirl the flask.

Storage conditions

Stored below -18 °C unopened internal standards are stable until the expiry date which is stated on the vial labels. The reconstituted internal standard can be stored up to 3 weeks tightly capped at +2 to +8 °C.