45000 HPLC kit homocysteine plasma serum

Homocysteine in Serum/Plasma - HPLC

Order no.: 45000
200 Tests
  • Homocysteine

Fast sample preparation
Short analysis time
Column life-time more than 2000 runs
Suitable for all blood collection systems


Clinical relevance

Homocysteine (Hcy) is a non-proteinogenic amino acid, synthesized during the demethylation of the amino acid methionine. Some enzymes are involved in this process, which require cofactors such as vitamin B6 and vitamin B12. A deficiency of these cofactors can lead to an increase in the Hcy concentration, but inherited genetic mutations may also be the cause.

Even moderately increased Hcy levels are a risk factor for cardiovascular diseases, arteriosclerosis and coronary heart diseases. Increased Hcy levels are also detected in the case of homocystinuria, an inherited genetic disorder in which the enzymes required for metabolism are not present or at levels that are too low. In this case, already newborns have high levels of Hcy, however, the disease is treatable if detected in time. Screening of newborns (NBS) for homocystinuria has therefore become mandatory in some European countries such as Ireland. In Germany it is only performed as part of more intensive screening or in cases where the disease is suspected.


Product advantages

  • Fast sample preparation
  • Short analysis time
  • Column lifetime of more than 2000 runs
  • For all blood collection systems


This reagent kit allows the simple and reliable determination of total homocysteine in serum/plasma. Sample preparation is simply a reduction step for releasing homocysteine from its protein binding, followed by precipitation and subsequent precolumn derivatisation. Chromatographic separation is performed on an isocratic HPLC system with fluorescence detection. The optimised reagents ensure the accurate and reliable quantification of total homocysteine for all blood collection systems within a brief analysis period of less than 6 minutes.


Technical Data

Method of Analysis


Number of Tests




Analysis Time

5.5 min

Limit of quantification

0.4 µmol/l


up to 400 µmol/l


96–99 %


CV = 0.9–2.2 %


CV = 2.9–3.1 %



Pre-analytic Treatment

Blood should be taken from the fasted patient. If no special homocysteine blood collecting systems are used, plasma/serum should be prepared within 45 min after drawing the sample. Homocystein in plasma is stable at room temperature for 24 h, at +4 °C up to 1 week and below -18 °C several months.

Sample Preparation

  • To 100 µl plasma/serum add 25 µl Internal Standard and 75 µl Reduction Reagent.
  • Shake for 2 s (vortex).
  • Incubate 10 min at ambient temperature.
  • Add 100 µl Precipitation Reagent.
  • Shake for 30 s (vortex).
  • Centrifuge 5 to 7 min at 9000 x g.
  • Pipette 100 µl derivatisation mix into a new light protected reaction vial.
  • Add 50 µl supernatant, mix well.
  • Incubate 10 min at +50 to +55 °C.
  • Cool down immediately.
  • Inject 20 to 50 µl into the HPLC system.

Sample Stability

The prepared samples are stable at ambient temperature up to 20 h, at +2 to +8 °C for 5 days and below -18 °C up to 3 weeks.

Additional Info

Any isocratic HPLC system with fluorescence detector is suitable.

Injection Volume

20–50 µl

Flow rate

1.3–1.7 ml/min

Column temperature

ambient (~ 25 °C)


EX 385 nm
EM 515 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately


Calibrators available separately


Controls available separately