59000 HPLC kit extended benzodiazepines serum plasma

Extended Benzodiazepines in Serum/Plasma - HPLC

Order no.: 59000
100 Tests
  • Bromazepam
  • Chlordiazepoxide
  • Clobazam
  • Clonazepam
  • Desalkylflurazepam
  • Diazepam
  • Flunitrazepam
  • Lorazepam
  • Medazepam
  • Midazolam
  • Nitrazepam
  • Nordiazepam
  • Oxazepam
  • Temazepam
  • Tetrazepam

6 additional analytes
Reduced run time
Custom-made internal standard

Description

Clinical relevance

Benzodiazepines have a broad range of applications and are the main representatives of sedatives and sleeping pills. Some are also used as antiepileptic drugs, muscle relaxants, and as analgesics in administering general anaesthesia. In the case of alcohol withdrawal treatments they facilitate weaning, although the danger of an alternative addiction exists. The various benzodiazepines are metabolised at different rates, and in some cases it is not until the metabolites form (e.g. desalkylflurazepam) become therapeutically effective.

Regular determination of the plasma levels minimise the risk of side effects resulting from overdose. Furthermore, patient compliance can be confirmed to help ensure optimal therapy outcomes.

 

Product advantages

  • All common benzodiazepines in a single run
  • Shorter analysis times
  • Custom-made internal standard

 

This Chromsystems kit allows for the easy and reliable chromatographic determination of a total of 15 benzodiazepines and metabolites. Sample preparation is based on an efficient and selective solid phase extraction, which can also be automated with the Gilson® ASPEC™. Chromatographic measurement is performed on an isocratic HPLC system with UV detection.

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

100

Parameter

Bromazepam, Chlordiazepoxide, Clobazam, Clonazepam, Desalkylflurazepam, Diazepam, Flunitrazepam, Lorazepam, Medazepam, Midazolam, Nitrazepam, Nordiazepam, Oxazepam, Temazepam, Tetrazepam

Analysis Time

< 26 min

Limit of quantification

6-11 µg/l

Linearity

from limit of quantification up to at least the stated upper limit

Recovery

94-103 %

Intraassay

CV = 0.9-4.8 %

Interassay

CV = 1.9-5.5 %

Information

Isocratic HPLC system with UV detector.

Column temperature

30 ± 2 °C

Flow rate

0,6 ml/min

Specimen

Plasma/Serum

Pre-analytic Treatment

Patient specimens are stable up to 1 week stored at +2 to +8 °C. For longer storage periods deep-freeze the patient specimens below -18 °C.

Sample Preparation

Extraction

  • Condition the Sample Clean Up Columns with 1 ml Equilibration Buffer 1 and 2 each.
  • Apply 1 ml plasma/serum and 100 µl Internal Standard, mix several times.
  • Draw through completely by centrifugation (1 min at 340 x g) or suction, discard effluent.

Wash

  • Apply 2 x 1 ml Wash Buffer to the Sample Clean Up Column and draw through by centrifugation (1 min at 340 x g) or suction, discard effluents. Then centrifuge 2 min at 1090 x g to dryness or use suction.

Elution

  • Apply 400 µl Elution Buffer to the Sample Clean Up Column, draw through completely by centrifugation or suction, then dilute with 300 µl distilled water.
  • Inject 50 µl of the diluted eluate into the HPLC system.

Sample Stability

At +2 to +8 °C up to 1 week. Freeze for longer storage.

Injection Volume

50 µl

Additional Info

Isocratic HPLC system with UV detector.

Wavelength

210 nm

Autosampler rinsing solution

water:acetonitrile = 75 : 25

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase


Order no.: 59001

Internal Standard


Order no.: 59004

Equilibration Buffer 1


Order no.: 59005

Equilibration Buffer 2


Order no.: 59006

Wash Buffer


Order no.: 59007

Elution Buffer


Order no.: 59008

Sample Clean Up Columns


Order no.: 49008

Calibrators

Controls