CDT in Serum - binary gradient systems - HPLC

CDT in Serum - binary gradient systems - HPLC

  • Asialotransferrin
  • Disialotransferrin
  • Pentasialotransferrin
  • Tetrasialotransferrin
  • Trisialotransferrin

Order no.: 54020, 54020/500
100 / 500 Tests

Reference method for CDT analyses: currently HPLC
Sample preparation: just 2 pipetting steps
Long column lifetime
Low cost per test


* Required Fields


Clinical relevance

CDT (carbohydrate-deficient transferrin) is a lab marker showing the highest specificity for chronic alcohol abuse. CDT values increase significantly with the daily intake of more than 60 g ethanol (the equivalent of 0.75 l wine) per day over a period of two weeks. The values return to normal within two weeks in case of alcohol abstention. This makes CDT an excellent parameter for continuous monitoring in withdrawal programmes or for assessments in forensic or occupational medicine.

Unlike other biochemical parameters like γ-GT or MCV, liver diseases will not cause any false-positive results.


Product advantages

  • Specific marker for chronic alcohol abuse
  • Long column lifetime
  • Low cost


This Chromsystems kit allows for the fast and reliable measurement of CDT in serum using HPLC and UV detection. The results are displayed as an area percentage of total transferrin, so they remain unaffected by fluctuations in transferrin concentration. Optimised chromatographic separation of transferrin isoforms also allows for the reliable detection of genetic variants of transferrin.

Alternative methods using pre-mixed tubes or automated sample preparation for higher sample throughput are also available.


Technical Data

Method of Analysis



Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin

Analysis Time

22 min

Limit of quantification

0.5 % Disialotransferrin


< 12 % Disialotransferrin


101 %


CV ≤ 7.7 %


CV ≤ 4.6 %


Serum (no anticoagulants, no EDTA or heparin plasma)

Pre-analytic Treatment

Time of sample drawing has no influence on the analysis, fasting serum preferred. Samples should be cooled during transport. Storage life is up to 1 week at +2 to +8 °C. For longer storage, deep-freeze below -18 °C.


Sample Preparation

  • Place 200 µl sample/control in a reaction vial.
  • Add 100 µl reaction mix*, mix for 60 s (vortex).
  • Incubate 30 min at +2 bis +8 °C.
  • Centrifuge 10 min at 9000 x g.
  • Dilute 200 µl supernatant with 400 µl ultrapure water (HPLC grade).
  • Inject 200 µl into the HPLC system.

* The reaction mix is prepared from Neutralisation Buffer, Stabilisation Buffer, Precipitation Reagent 1 and 2 (25 µl each per sample). Independent of the number of specimens you are analyzing you can prepare this "master mix" only once and can add it to the samples e.g. by a multipette.

Sample Stability

Samples are stable at room temperature up to 30 h, at +2 to +8 °C up to 1 week. For longer storage keep them frozen below –18 °C.

Additional Info

For the Chromsystems HPLC analysis of CDT in serum any binary HPLC gradient system with UV detection is suitable.

Injection Volume

200 µl

Flow rate

1.5 ml/min (analysis); 2.0 ml/min (column wash)



Column temperature

ambient (~25 °C)


460 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Mobile Phase A

Order no.: 54021

Mobile Phase B

Order no.: 54022

Neutralisation Buffer

Order no.: 54025

Stabilisation Buffer

Order no.: 54026

Precipitation Reagent 1

Order no.: 54027

Precipitation Reagent 2

Order no.: 54028

Reaction Vials

Order no.: 3006


Controls available separately