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CDT FE in Serum Fast Elution - HPLC

Order no.: 54330/500
500 Tests
  • Asialotransferrin
  • Disialotransferrin
  • Pentasialotransferrin
  • Tetrasialotransferrin
  • Trisialotransferrin

Short analysis time
Detects genetic variants
Traceable to the IFCC reference measurement procedure

Description

Clinical relevance

CDT and in particular disialotransferrin is regarded as the most specific and most sensitive laboratory marker of chronic alcohol abuse. A daily intake of more than 60 g ethanol (about 0.75 l wine) over a longer period elevates CDT values significantly. These return to normal after alcohol abstinence. This makes CDT an excellent parameter for controlling withdrawal treatment, forensic assessment or in occupational medicine.

 

Product advantages

  • Specific marker for chronic alcohol abuse
  • Run time of less than 10 min
  • Detection of genetic variants
  • Traceable to the IFCC reference measurement procedure

 

This Chromsystems kit is designed for the specific and fast determination of CDT in serum with a ternary gradient HPLC system. During sample preparation, lipoproteins are removed by precipitation with simultaneous iron saturation. The chromatography enables a very good separation of asialo-Tf, disialo-Tf and trisialo-Tf in less than 6 minutes. The transferrins are separated using two mobile phases, thereafter the column is cleaned with a third mobile phase.

This method is traceable to the IFCC reference measurement procedure (IFCC-RMP) and certified accordingly. It can therefore also be used to determine CDTIFCC. The IFCC certificate is available for customers in the secure download area.

Chromatogram

Technical Data

Method of Analysis

HPLC

Number of Tests

500

Parameter

Asialotransferrin, Disialotransferrin, Pentasialotransferrin, Tetrasialotransferrin, Trisialotransferrin

Analysis Time

9.5 min

Limit of quantification

approx. 0.5 % Disialotransferrin

Linearity

up to at least 12 % Disialotransferrin

Intraassay

CV = 5.7 %

Interassay

CV = 6.3 %

Specimen

Serum (no anticoagulants, no EDTA or heparin plasma)

Pre-analytic Treatment

Specimens should be kept cool for transport. Storage life is up to 1 week at +2 to +8 °C. For longer storage, deep-freeze below –18°C.

Sample Preparation

  • Place 200 µl sample/control into a reaction vial.
  • Add 100 µl reagent mix*, mix for 60 s (vortex).
  • Incubate for 30 min at +2 bis +8 °C.
  • Centrifuge for 10 min at 9000 x g.
  • Dilute 200 µl supernatant with 400 µl ultrapure water (HPLC grade).
  • Inject 150 µl into the HPLC system.

* The reaction mix is prepared from Neutralisation Buffer, Stabilisation Buffer, Precipitation Reagent 1 and 2 (25 µl each per sample)

Sample Stability

At ambient temperature up to 30 h. Keep cool for longer transport and deep-freezing is acceptable.

Additional Info

For the Chromsystems HPLC analysis of CDT FE in serum (Fast Elution) a ternary HPLC gradient system with UV detection is necessary.

Injection Volume

100-150 µl

Flow rate

2.5 ml/min

Gradient

Ternary

Column temperature

+30 °C

Wavelength

460 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

Kit Components

Components available separately

Neutralisation Buffer


Order no.: 54025

Stabilisation Buffer


Order no.: 54026

Precipitation Reagent 1


Order no.: 54027

Precipitation Reagent 2


Order no.: 54028

Reaction Vials


Order no.: 3006

Controls

Controls available separately