6PLUS1® Multilevel Urine Calibrator Set VMA, HVA, 5-HIAA

6PLUS1® Multilevel Urine Calibrator Set VMA, HVA, 5-HIAA

Order no.: 80839
  • 5-Hydroxyindoleacetic acid (5-HIAA)
  • Homovanillic acid (HVA)
  • Vanillylmandelic acid (VMA)

MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

Description

6PLUS1® Multilevel Urine Calibrator Set VMA, HVA, 5-HIAA, lyophilised

For MassChrom® Biogenic Amines/Metabolites in Urine with Autosampler Vials or Collection Plates.

These lyophilised calibrators from Chromsystems are based on human urine and should be used according to the instruction manual. They are designed to calibrate the measurement method in clinical diagnostics for the quantitative determination of vanillylmandelic acid (VMA), homovanillic acid (HVA) and 5-hydroxyindole acetic acid (5-HIAA) in urine. While following the instructions the calibrators should be handled and measured in the same manner as a patient specimen.
These calibrators are only available as multilevel calibrator set with 6 different concentration levels plus 1 blank value.

Technical Data

Method of Analysis

LC-MS/MS

Content

7 x 1 ml (lyoph.)

Parameter

5-Hydroxyindoleacetic acid (5-HIAA), Homovanillic acid (HVA), Vanillylmandelic acid (VMA)

Concentrations

Please note: Concentration values shown here are typical. For lot specific concentration values please refer to the product insert supplied with the materials. The product inserts can also be requested from us directly or downloaded from our website after login.

 
Substance UnitCalibrator 1Calibrator 2Calibrator 3Calibrator 4Calibrator 5Calibrator 6Blank Calibrator
VMA (Vanillylmandelic Acid) mg/l 
µmol/l
1.19
6.00
9.37
47.3
17.4
87.8
25.4
128
51.1
258
77.3
390
n.d.
HVA (Homovanillic Acid) mg/l 
µmol/l
0.869
4.77
11.8
64.8
23.4
128
34.7
190
79.9
439
119
653
n.d.
5-HIAA (5-Hydroxyindoleacetic Acid) mg/l 
µmol/l
0.699
3.66
2.51
13.1
4.55
23.8
6.44
33.7
37.2
195
68.0
356
n.d.

n.d. = not determined (< LOQ)

Reconstitution

Carefully remove the metal seal and rubber stopper from the vials. Add exactly 1.0 ml of distilled water into each vial. Return the stopper and incubate for 10 to 15 minutes at room temperature. Swirl the vials to dissolve the contents until homogeneity.

Storage conditions

Stored below -18 °C and unopened, the lyophilised calibrators are stable until the expiry date specified on the vial labels. The reconstituted calibrators can be stored up to 4 weeks light protected and tightly capped at +2 to +8 C. If the calibrators cannot be used within this period, they should be aliquoted and stored frozen below -18 °C (up to 3 months). For more details about storage conditions please check your instruction manual.